It has been described that the antioxidant enzyme systems play an important role in the control of apoptosis and that the apoptogenic ability of TGF-β1 is through the inhibition of antioxidant enzyme expression in cultured hepatocytes [Y. Kayanoki, J. Fujii, K. Suzuki, S. Kawata, Y. Matsuzawa and N. Taniguchi, Suppression of antioxidative enzyme expression by transforming growth factor-β 1 in rat …
Effect of phorbol ester (PMA) on antioxidant enzyme expression in TGF- β1-induced apoptosis in primary cultures of hepatocytes
Read «Effect of phorbol ester (PMA) on antioxidant enzyme expression in TGF- \beta 1-induced apoptosis in primary cultures of hepatocytes, BioFactors» on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Moreover, suppression of antioxidant enzyme gene expression, together with the appearance of oxidative stress, is involved in the process of TGF-β1 induction of apoptosis , . Nuclear factor-κB (NF-κB), an inducible eukaryotic transcription factor of the rel family, normally exists in an inactive cytoplasmic complex, bound to inhibitory proteins of the IκB family.
The effect of α-lipoate pretreatment on PMA-induced expression of VCAM-1 in ECV cells was determined . The constitutive expression of VCAM-1 in ECV cells was observed to be very low. PMA-induced VCAM-1 expression was down-regulated by α-lipoate treatment in a dose dependent manner.
Aug 15, 1989 · However, after a 5 min pre-treatment, thrombin-induced secretion alone was inhibited, whereas PMA plus GTP[S]/NaF-induced release remained greater than additive. [32P]Phosphatidate formation in response to all three agents, in contrast, was inhibited by …
The effect of non-genotoxic carcinogens, phenobarbital and clofibrate, on the relationship between reactive oxygen species, antioxidant enzyme expression and apoptosis
Dec 25, 1986 · Like insulin, PMA provokes a concentration and time-dependent decrease of mRNA coding for that enzyme that is due to an inhibition of P-enolpyruvate carboxykinase gene transcription. This effect of PMA is rapid, reversible, specific for phorbol esters known to be active in other systems, and it does not require on-going protein synthesis.
Protein kinase C-zeta (PKC-zeta) is a member of the protein kinase C gene family which using in vitro preparations has been described as being resistant to activation by phorbol esters. PKC-zeta was found to be expressed in several cell types as an 80-kDa protein.
Since PMA is known to cause cytosol-to-membrane shift of calcium-activated, phospholipid-dependent protein kinase (protein kinase c, PKC) in human neutrophils, we investigated the role of PAF in modifying PMA-induced PKC activation/translocation.
First, both phorbol myristate acetate (PMA) and diacylglycerol, 2 activators of protein kinase C (PKC), were found to stimulate calcium entry into RBCs, whereas 4α-phorbol ester, an …
Abstract. We studied the ability of norepinephrine to stimulate [3H]inositol trisphosphate production and calcium mobilization in rat-1 fibroblasts stably expressing the cloned alpha 1-adrenergic subtypes and their sensitivity to phorbol-12-myristate-13-acetate (PMA).
CD28 is a costimulatory receptor found on the surface of most T lymphocytes. Engagement of CD28 induces interleukin 2 (IL-2) production and cell proliferation when combined with an additional signal such as treatment with phorbol ester, an activator of protein kinase C.